Related Experiment Video
Updated: Aug 13, 2026

06:16
Cryosectioning Method for Microdissection of Murine Colonic Mucosa
Published on: July 12, 2015
Dissociation of rat colonic mucosa into single epithelial cells and their microscopic visualization
Abstract:
Repeated incubation of rat colonic mucosa with phosphate-buffered mannitol yields morphologically intact single cell suspensions which are easily visualized microscopically with trypan blue. Cell dimensions are determined by means of a Nikon microcomparator.

