Related Experiment Videos
[A fast and simple micromethod for estimating proteins]
Acta Biologica Et Medica Germanica
|January 1, 1979
Summary
This study presents a protein assay utilizing bromophenol blue complexation for accurate quantification. The method is sensitive and adaptable for measuring both small and large protein concentrations.
Area of Science:
- Biochemistry
- Analytical Chemistry
Context:
- Protein quantification is essential in biological research.
- Existing methods may require larger sample volumes or lack sensitivity for trace amounts.
Purpose:
- To develop a sensitive and versatile protein assay.
- To enable accurate protein measurement in small sample volumes.
Summary:
- The assay leverages the complexation of proteins with bromophenol blue in an alkaline solution.
- The resulting blue color is quantifiable spectrophotometrically at 598 nm.
- The method is optimized for 5-50 microliter volumes containing 5-30 micrograms of protein, with modifications for higher concentrations.
Impact:
- Provides a reliable method for protein determination in limited sample sizes.
- Facilitates protein analysis in various biochemical and clinical applications.
- Offers a cost-effective and accessible protein quantification technique.