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Related Experiment Videos

Is the L/S ratio really a lecithin/sphingomyelin ratio?

C G Duck-Chong, L M Brown

    Annals of Clinical Biochemistry
    |July 1, 1979
    PubMed
    Summary

    Assessing fetal lung maturity via the lecithin/sphingomyelin ratio is complicated by interfering phospholipids like phosphatidylserine and phosphatidylinositol in amniotic fluid, causing significant variations in results.

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    Area of Science:

    • Obstetrics and Gynecology
    • Clinical Chemistry
    • Neonatal Medicine

    Background:

    • Fetal lung maturity assessment is crucial for optimizing delivery timing.
    • The lecithin/sphingomyelin ratio in amniotic fluid is a standard method for this assessment.
    • Current chromatographic methods face challenges with interfering substances.

    Purpose of the Study:

    • To investigate interfering phospholipids in amniotic fluid affecting the lecithin/sphingomyelin ratio.
    • To identify the nature of these interfering phospholipids.
    • To evaluate the effectiveness of pre-chromatographic treatment in removing interferences.

    Main Methods:

    • Analysis of amniotic fluid phospholipids using various chromatographic systems.
    • Identification of phospholipids co-eluting with lecithin and sphingomyelin.
    • Evaluation of cold acetone treatment on lipid extracts.

    Main Results:

    • Phosphatidylserine and phosphatidylinositol were identified as major interfering phospholipids.
    • These phospholipids chromatograph between or overlap with lecithin and sphingomyelin.
    • Cold acetone treatment did not effectively remove these interfering substances.

    Conclusions:

    • The presence of phosphatidylserine and phosphatidylinositol significantly impacts the accuracy of the routine lecithin/sphingomyelin ratio.
    • Interfering phospholipids introduce considerable variability in fetal lung maturity assessments.
    • Standard chromatographic methods require refinement to improve the reliability of this diagnostic test.

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