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Related Experiment Videos

Neural cells in culture.

R M Mulligan, C M Carnes

    Annals of Clinical and Laboratory Science
    |September 1, 1979
    PubMed
    Summary

    Neural cells in vitro exhibit a novel non-mitotic replication after 30 days. New cells bud from process ends, containing nuclei and mitochondria, indicating a unique developmental pathway.

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Developmental Biology

    Background:

    • Neural cells, including nerve sheath cells, melanoblasts, neuroblasts, and astrocytes, were cultured in vitro.
    • Previous understanding of neural cell replication primarily focused on mitotic processes.

    Purpose of the Study:

    • To investigate the in vitro replication mechanisms of various neural cell types.
    • To characterize the morphological and developmental changes during prolonged neural cell culture.

    Main Methods:

    • In vitro cell culture of neural cells for 3 to 20 months.
    • Phase contrast microscopy, differential interference electron microscopy, and time-lapse cinemicrophotography.
    • Detailed morphological analysis of cell bodies, nuclei, nucleoli, and cellular processes.

    Main Results:

    • A non-mitotic replication pattern emerged between 30 and 120 days in culture.
    • Neural cells transitioned from unipolar/bipolar to multipolar/dendritic forms with complex processes.
    • New cells formed via budding from process terminals, containing nuclei and mitochondria, after a unique process involving mitochondrial transport and granule formation.

    Conclusions:

    • Neural cells can undergo a distinct non-mitotic form of replication in vitro.
    • This process involves the formation of specialized structures at process terminals for generating new cells.
    • The findings suggest a previously undescribed mechanism for neural cell proliferation and development.

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