Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbubbles in replicating nuclear deoxyribonucleic acid from Physarum polycephalum.

D A Gillespie, N Hardman

    The Biochemical Journal
    |November 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    AKT overactivation can suppress DNA repair via p70S6 kinase-dependent downregulation of MRE11.

    Oncogene·2017
    Same author

    PERK inhibits DNA replication during the Unfolded Protein Response via Claspin and Chk1.

    Oncogene·2016
    Same author

    Chk1 is essential for chemical carcinogen-induced mouse skin tumorigenesis.

    Oncogene·2011
    Same author

    Cdk-mediated phosphorylation of Chk1 is required for efficient activation and full checkpoint proficiency in response to DNA damage.

    Oncogene·2011
    Same author

    Chk1 C-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of Chk1 catalytic activity.

    Oncogene·2009
    Same author

    v-Jun sensitizes cells to apoptosis by a mechanism involving mitochondrial cytochrome C release.

    Oncogene·2000

    Researchers found microbubble clusters in Physarum polycephalum DNA, indicating active DNA synthesis during S-phase. These clusters may represent DNA replicons, crucial units for DNA replication.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Genetics

    Background:

    • DNA replication is a fundamental process for cell division.
    • Understanding the initiation sites of DNA synthesis is key to comprehending genome duplication.
    • Physarum polycephalum serves as a model organism for studying nuclear processes.

    Purpose of the Study:

    • To identify and characterize structures associated with the initiation of DNA synthesis in Physarum polycephalum.
    • To investigate the relationship between microbubble clusters and DNA replication.
    • To determine the potential role of these structures as replicons.

    Main Methods:

    • Electron microscopy was used to visualize nuclear DNA from Physarum polycephalum.
    • Specific sample preparation techniques were employed to identify microbubble clusters.

    Related Experiment Videos

  • Analysis of microbubble size and association with S-phase was performed.

    • Main Results:

    • Clusters of microbubbles, indicative of newly initiated DNA synthesis, were observed in Physarum polycephalum nuclear DNA.
    • The presence of these microbubbles was specifically correlated with the S-phase of the cell cycle.
    • Each microbubble cluster was estimated to correspond to a DNA segment of 100-5000 nucleotide residues, consistent with a replication unit.
    • These DNA structures were found to be metastable, reverting to native DNA under specific preparation conditions.

    Conclusions:

    • Microbubble clusters in Physarum polycephalum DNA are likely sites of DNA replication initiation.
    • The observed clusters are proposed to represent functional replicons, the basic units of DNA replication.
    • This finding provides insights into the mechanism of DNA synthesis regulation in eukaryotes.