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Human colostral cells. I. Separation and characterization.

S S Crago, S J Prince, T G Pretlow

    Clinical and Experimental Immunology
    |December 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

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    Human colostrum contains approximately 3.3 million cells per ml, primarily macrophages and polymorphonuclear leukocytes. These phagocytic cells ingest lipids and milk proteins, but lymphoid cells do not.

    Area of Science:

    • Immunology
    • Cell Biology
    • Lactation Biology

    Background:

    • Human colostrum is a crucial source of immune factors for newborns.
    • Understanding the cellular composition and function of colostrum is vital for infant health.
    • Previous characterization of colostral cells has been limited.

    Purpose of the Study:

    • To comprehensively analyze the cell types and their protein content in early human colostrum.
    • To investigate the origin and function of proteins within colostral cells.
    • To evaluate the reliability of standard lymphocyte identification markers in colostrum.

    Main Methods:

    • Analysis of colostrum from 54 healthy donors using histochemical and immunohistochemical staining.
    • Cell separation techniques including density centrifugation and adherence.

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  • Detection of specific proteins (immunoglobulins, secretory component, lactoferrin, alpha-lactalbumin) and lipids within cells.

    • Main Results:

    • Colostrum contains 3.3 x 10^6 cells/ml, predominantly macrophages (30-47%) and polymorphonuclear leukocytes (40-60%).
    • Phagocytic cells (macrophages, polymorphonuclear leukocytes, colostral corpuscles) contain lipids and various milk proteins, acquired through ingestion.
    • Standard lymphocyte markers were unreliable; lymphocytes were classified as T cells based on association with other cells.

    Conclusions:

    • Human colostrum harbors a significant population of phagocytic cells actively involved in protein and lipid uptake.
    • The presence of ingested proteins within phagocytic cells highlights their role in nutrient and immune transfer.
    • Morphological characterization is essential for accurate cell identification in colostrum due to limitations of standard markers.