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Related Experiment Videos

Platelet counting-development of a reference method and a reference preparation.

S M Lewis, J Wardle, S Cousins

    Clinical and Laboratory Haematology
    |January 1, 1979
    PubMed
    Summary
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    A new method using platelet-rich plasma (PRP) and an electronic counter offers a reliable and rapid approach for platelet counting, comparable to standard reference methods. This technique can serve as both a secondary reference and routine method for accurate blood cell analysis.

    Area of Science:

    • Hematology
    • Clinical Pathology
    • Laboratory Medicine

    Background:

    • Accurate platelet counting is crucial for diagnosing and managing various hematological disorders.
    • Existing reference methods for platelet counting can be time-consuming and complex.
    • There is a need for reliable, rapid, and simple methods for routine platelet enumeration.

    Purpose of the Study:

    • To develop and validate a new method for platelet counting using platelet-rich plasma (PRP) and an electronic counter.
    • To establish a secondary reference method and a routine method for platelet counting.
    • To identify an optimal method for obtaining platelet-rich plasma from EDTA-treated blood.

    Main Methods:

    • Developed a procedure using platelet-rich plasma (PRP) and a Coulter ZBI counter for platelet counting.

    Related Experiment Videos

  • Optimized PRP isolation from EDTA blood using augmented sedimentation with a specific methyl cellulose-metrizoate mixture (sp. gr. 1.08).
  • Investigated the suitability of glutaraldehyde-fixed human platelets in glycerol as a stable reference preparation for calibrating electronic counters.

    • Main Results:

    • The developed PRP method using the Coulter ZBI counter is reliable, rapid, and comparable to the reference counting chamber haemocytometry method.
    • Augmented sedimentation with the specified mixture yielded a platelet suspension accurately reflecting the original blood's platelet content.
    • A suspension of glutaraldehyde-fixed human platelets in glycerol was found to be a suitable reference material for electronic counter calibration.

    Conclusions:

    • The developed PRP-based electronic counting method is suitable as a secondary reference and routine method for platelet counting.
    • The optimized PRP isolation technique ensures accurate representation of platelet counts.
    • Glutaraldehyde-fixed human platelets in glycerol provide a stable and comparable reference preparation for calibrating automated platelet counters.