Jove
Visualize
Contact Us

Related Experiment Videos

Improved screening test for abnormal hemoglobins from dried blood samples.

K Altland, M Kaempfer, H Granda

    Human Genetics
    |January 1, 1979
    PubMed
    Summary

    Dried blood spot analysis enables hemoglobin chain separation for up to two years post-collection. This method is valuable for sickle cell anemia screening and human mutation monitoring.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Study of KIR genes in tuberculosis patients.

    Tissue antigens·2006
    Same author

    Leptomeningeal familial amyloidosis: A rare differential diagnosis of leptomeningeal enhancement in MRI.

    Journal of neurology·2006
    Same author

    "Sporadic" familial amyloidotic polyneuropathy in a German patient with B cell lymphocytic leukaemia.

    Journal of neurology, neurosurgery, and psychiatry·2002
    Same author

    A distinct gene close to the hairless locus on chromosome 8p underlies hereditary Marie Unna type hypotrichosis in a German family.

    The British journal of dermatology·2000
    Same author

    Transthyretin Val71Ala mutation in a Dutch family with familial amyloidotic polyneuropathy.

    Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis·2000
    Same author

    Potential treatment of transthyretin-type amyloidoses by sulfite.

    Neurogenetics·1999
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Biochemistry
    • Genetics
    • Clinical Diagnostics

    Background:

    • Dried blood spots are a convenient sample matrix for various analyses.
    • Accurate hemoglobin chain analysis is crucial for diagnosing hemoglobinopathies and monitoring genetic mutations.

    Purpose of the Study:

    • To develop and validate a method for analyzing hemoglobin chains from dried blood spots.
    • To assess the stability and utility of dried blood samples for hemoglobin analysis over time.

    Main Methods:

    • Blood samples dried on filter paper were eluted using 8 M urea, 5% 2-mercaptoethanol, and 2% Nonidet P-40.
    • Individual hemoglobin chains (alpha, beta, gamma, epsilon) were separated using electrofocusing in 8 M urea-polyacrylamide gel electrophoresis (PAA).
    • High-throughput analysis was achieved with multiple syringes for sample application and daily processing of several hundred samples.

    Main Results:

    • The described method allows for reliable hemoglobin chain separation from dried blood spots.
    • Hemoglobin analysis is feasible for samples stored up to two years.
    • The technique supports the analysis of up to 96 samples per gel, enabling high-throughput screening.

    Conclusions:

    • This method provides a stable and efficient approach for hemoglobin chain analysis using dried blood spots.
    • It is particularly suitable for large-scale preventive programs, such as sickle cell anemia screening.
    • The technique can be applied to human mutation monitoring systems, aiding in genetic research and diagnostics.

    Related Experiment Videos