Related Experiment Video

Updated: Aug 8, 2026

Preparing T Cell Growth Factor from Rat Splenocytes
18:27

Preparing T Cell Growth Factor from Rat Splenocytes

Published on: November 1, 2007

Inhibition of mitogen induced blast transformation by a multiple sclerosis serum factor

J A Anderson, P Sweet, S A Armentrout

    Journal of Clinical & Laboratory Immunology
    |November 1, 1979
    PubMed

    Insights

    A factor found in multiple sclerosis patient sera inhibits RNA, protein, and DNA synthesis in stimulated lymphocytes, preceding cell death. This factor affects committed or simultaneously exposed cells but not unstimulated ones.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Biology

    Background:

    • Multiple Sclerosis (MS) is an autoimmune disease affecting the central nervous system.
    • Lymphocyte dysfunction is implicated in the pathogenesis of MS.
    • Understanding molecular mechanisms in MS pathogenesis is crucial for developing targeted therapies.

    Purpose of the Study:

    • To investigate the effect of a serum factor from MS patients on lymphocyte macromolecular synthesis.
    • To determine the impact of this factor on lymphocyte viability and response to mitogens.

    Main Methods:

    • Isolation of lymphocytes from healthy donors.
    • Stimulation of lymphocytes with mitogens.
    • Incubation of lymphocytes with MS patient serum factor.
    • Measurement of RNA, protein, and DNA synthesis.
    • Assessment of cell viability and response to subsequent mitogen stimulation.

    Main Results:

    • A serum factor from MS patients significantly inhibits RNA, protein, and DNA synthesis in mitogen-stimulated lymphocytes.
    • Inhibition of macromolecular synthesis occurs 24–36 hours before observable cell death.
    • The factor impacts lymphocytes already committed to transformation or simultaneously exposed to mitogen.
    • Unstimulated lymphocytes are not affected by the factor, and their subsequent response to mitogens remains unaltered.
    • The factor does not inhibit RNA synthesis or exhibit toxicity in unstimulated lymphocytes.

    Conclusions:

    • A specific serum factor in MS patients can suppress key cellular processes in activated lymphocytes.
    • This inhibition of macromolecular synthesis may contribute to lymphocyte dysfunction observed in MS.
    • The factor's targeted action on stimulated cells suggests a role in modulating immune responses during MS flares or progression.

    More Related Videos

    Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs08:13

    Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

    Published on: December 27, 2016

    Adipose-Derived Mesenchymal Stromal Cells Co-Cultured with Primary Mixed Glia to Reduce Prion-Induced Inflammation10:40

    Adipose-Derived Mesenchymal Stromal Cells Co-Cultured with Primary Mixed Glia to Reduce Prion-Induced Inflammation

    Published on: August 11, 2023

    Related Concept Videos

    Multiple Sclerosis l: Introduction01:19

    Multiple Sclerosis l: Introduction

    Multiple sclerosis is a chronic autoimmune disease of the central nervous system (CNS) that affects the brain, spinal cord, and optic nerves. It is an inflammatory demyelinating disorder and a leading cause of neurological disability in young adults.EpidemiologyMS commonly begins between 20 and 40 years of age and is twice as common in women. Its exact cause remains unclear, but genetic susceptibility contributes, with higher risk in first-degree relatives and identical twins. A greater...