Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple active sites on human interferons.

K Paucker, B J Dalton, C A Ogburn

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1975
    PubMed
    Summary

    Human interferons from leukocytes and fibroblasts exhibit distinct antiviral activities in rabbit cells. These interferons possess unique molecular structures enabling interaction with different host cells, explaining varied antiviral protection.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Comparison of in vitro and in vivo labeling of virus-induced L-cell interferon.

    The Journal of general physiology·2009
    Same author

    A modified caprylic acid method for manufacturing immunoglobulin G from human plasma with high yield and efficient virus clearance.

    Vox sanguinis·2006
    Same author

    Viral safety of Nanogam, a new 15 nm-filtered liquid immunoglobulin product.

    Vox sanguinis·2005
    Same author

    Cholangiography during laparoscopic cholecystectomy--cumulative sum analysis of an institutional learning curve.

    Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·1999
    Same author

    Patterns of morbidity and resource consumption associated with laparoscopic cholecystectomy in a VA medical center.

    The Journal of surgical research·1999
    Same author

    Microtiter plate assay for measuring the anticomplementary activity of immunoglobulins.

    Journal of immunological methods·1993

    Area of Science:

    • Immunology
    • Virology
    • Molecular Biology

    Background:

    • Human interferons (IFNs) are crucial for antiviral defense.
    • IFNs produced by different cell types (leukocytes, fibroblasts) may have distinct properties.
    • Understanding IFN cross-species activity is vital for therapeutic applications.

    Purpose of the Study:

    • To investigate the antigenic and functional properties of human leukocyte and fibroblast interferons.
    • To determine the molecular basis for the antiviral activity of human IFNs in rabbit cells.
    • To propose a model explaining differential antiviral protection.

    Main Methods:

    • Antigenic characterization using neutralization tests with anti-interferon sera.
    • Affinity chromatography using Sepharose-bound antibodies.
    • Antiviral activity assays in human and rabbit cell cultures.

    Main Results:

    • Human leukocyte and fibroblast IFNs show distinct antiviral activities in rabbit cells.
    • Leukocyte IFN contains a minor component with fibroblast-specific antigenic determinants.
    • Antiviral activities in both human and rabbit cells are linked to single IFN molecules.

    Conclusions:

    • Human IFNs possess multiple reactive sites, allowing interaction with diverse host cells.
    • Variations in determinant sites explain differential antiviral protection across species.
    • This suggests a flexible molecular mechanism for interferon's broad-spectrum antiviral action.

    Related Experiment Videos