Related Experiment Video
Updated: Aug 4, 2026

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
Published on: March 8, 2013
[Lymphographic visualization of the rectal lymphatic channels and nodes]
Abstract:
Injection of Lipiodol U.F. was effected in the submucosa of the rectum. Injection was done exactly 1,6 mm into the wall. This created a wheal from which reflux of the contrast medium occured. Up to 5 ml Lipiodol U.F. were injected in this manner. Roentgenograms were made in two planes after 2,4,8,24 and 48 hours; occasionally, oblique or spotfilm exposures were taken. Accumulation of the contrast medium began after only 2 to 4 hours, usually reaching its maximum after 12 hours. Thereafter, no further lymph nodes became apparent, so that radiographs taken at this time are representative of final stage. This procedure was carried out on 12 patients suffering from rectum carcinoma and on one patient with a villous adenoma. All tumors were located at a depth of 4 to 6 cm. The results are demonstrated by pictures. The lymph was found to flow longetudinally in the wall of the rectum. The order of appearence of the nodes was arbitrary. Due to the small size of the lymph nodes, no preoperative conclusion could be drawn as to wether or not they had become metastatically infested. The postoperative radiographs provided a check in the radicality of the operative procedure, especially in those cases in which lymph nodes had been clearly visible. Because of the viscosity of the contrast medium and because of the quantity that remained in the submucosa and was resorbed by the lymph channels, the method was self-limiting.
Related Concept Videos
Lymphatic Vessels and Lymph Transport
This one-way system allows fluids, solutes, and even pathogens to enter but prevents their return to the intercellular spaces.
Development of the Lymphatic System
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
Assessment of the Rectum and Anus
Rectal Inspection
Begin by inspecting the perianal and anal areas for color, texture, rashes,...
Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and solid...

