[Chromosome aberrations in oncobiogram - cytogenetic studies into aberration frequency following trenimon treatment...

Quantitative determination of qualitatively unequivocal signs for an individual oncobiogram is difficult. However, the use of a properly standardised analysis of chromosomes may offer a possible approach to the problem. Sixty-nine tissue cultures were prepared from 15 ovarian carcinomas, and 56 of them under went sufficient growth. A concentration of 2 . 10(-7) moles/l of trenimon was added to the tissue culture for one hour. Sixteen tissue cultures were used as controls. Chromosome preparation was achieved with good success after recovery periods between five and nine hours. A total of 2,890 mitoses was evaluated. Trenimon increased the amount of damaged mitoses from 1.2 per cent to eleven per cent, while the number of aberrations was aggravated even by the factor of 6.6. - The differences were statistically secured with high significance. Some of the cell cultures suffered rises in aberration frequency by 50 per cent and even 190 per cent. Such extreme values obviously suggest the presence of extraordinary sensitivity which be utilised for cytogenetic oncobiogram.