Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amino acid utilization patterns in clostridial taxonomy.

S R Elsden, M G Hilton

    Archives of Microbiology
    |November 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Volatile acid in the digesta of ruminants and other animals.

    The Journal of experimental biology·2010
    Same author

    The oxygen consumption of the sheep foetus.

    The Journal of physiology·2010
    Same author

    Volatile fatty acids in the rumen of the sheep.

    The Proceedings of the Nutrition Society·2010
    Same author

    The fermentation of carbohydrates in the rumen of the sheep.

    The Journal of experimental biology·2010
    Same author

    Discussion on digestion in the ruminant.

    Proceedings of the Royal Society of Medicine·2010
    Same author

    Carbon dioxide utilization in the formation of glycine and acetic acid.

    The Journal of biological chemistry·2010

    This study adapted polyamide chromatography for analyzing microbial amino acid metabolism. Different Clostridium species exhibited unique amino acid utilization and production patterns, aiding in their physiological classification.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Understanding microbial metabolism is crucial for various scientific fields.
    • Amino acid profiles in growth media can reveal metabolic activities of microorganisms.
    • Chromatographic techniques are essential for analyzing complex biological mixtures.

    Purpose of the Study:

    • To adapt the polyamide layer technique for qualitative amino acid analysis in microbial culture media.
    • To investigate the amino acid metabolism of various proteolytic Clostridia species.
    • To determine if amino acid profiles can be used for classifying Clostridia.

    Main Methods:

    • Adapted polyamide layer chromatography for separating dimethylaminonaphthalene sulphonyl amino acids.
    • Analyzed amino acid composition of culture media before and after microbial growth.

    Related Experiment Videos

  • Utilized casein hydrolysate, yeast extract, and trypticase as growth media components.

    • Main Results:

    • Identified specific amino acid consumption and production by different Clostridia species.
    • Clostridium species showed distinct patterns in proline reduction and 2-aminobutyric acid production.
    • Observed unique metabolic pathways, including the oxidation of aromatic amino acids and production of ornithine.

    Conclusions:

    • The adapted chromatographic method effectively differentiates Clostridia based on their amino acid metabolism.
    • Amino acid profiles provide valuable insights into the physiological grouping and species-specific characteristics of Clostridia.
    • This technique aids in the qualitative analysis of microbial metabolic pathways.