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[Behavioral and EEG effects of Zingiber mioga Roscoe (water soluble fraction) (author's transl)]
Abstract:
Behavioral and EEG effects of water soluble fraction extracted from Zingiber mioga Roscoe (Z. mioga) were investigated in mice, rats and rabbits. Z. mioga was dissolved in isotonic saline and given i.p. into mice and rats, and i.v. into rabbits, respectively. Z. mioga at doses of 50 approximately 400 mg/kf produced a marked reduction in locomotor activity of mice in the open-field test. The peak time of reduction was 30 min after the injection. In mice, Z. mioga at the same doses lowered significantly the rectal temperature and prolonged the sleeping time induced by thiopental-Na (40 mg/kg, i.v.). Z. mioga at a dose of 400 mg/kg had no anticonvulsant effects and it produced a slight muscle relaxation in mice. Z. mioga at doses of 50 approximately 200 mg/kg induced a significant inhibition of the active conditioned avoidance response of the rat in a shuttle box without affecting the unconditioned escape response. In the step-down method, however, the passive conditioned avoidance response was rarely affected by Z. mioga, but the response was suppressed by chlorpromazine. In rabbits with chronically implanted electrodes, Z. mioga at doses of 20--30 mg/kg induced a drowsy pattern of spontaneous EEG, ilel high voltage slow waves in the neocortex and amygdala, and desynchronization in the case of hippocampal theta waves. The EEG arousal response to the external auditory stimulation was inhibited by the same doses of Z. mioga, whereas it failed to suppress the arousal response to either the midbrain reticular or posterior hypothalamic stimulation, as with chlorpromazine. Neither the recruiting response nor the photic driving response were affected by Z. mioga. On the other hand, the limbic after-discharges to the hippocampal or amygdaloid stimulation were enhanced by Z. mioga as well as chlorpromazin, but they were inhibited by diazepam. The EEG effects of Z. mioga were qualitatively similar to chlorpromazine, but not to diazepam. These results indicate the similarity of behavioral and EEG effects of Z. mioga to those seen with neuroleptics such as chlorpromazine.
Insights
The water-soluble fraction of Zingiber mioga (Z. mioga) reduced mouse locomotor activity and prolonged sleep. Z. mioga exhibited neuroleptic-like behavioral and EEG effects, similar to chlorpromazine.
Area of Science:
- Pharmacology
- Neuroscience
- Ethnobotany
Background:
- Zingiber mioga Roscoe (Z. mioga) is a plant with traditional medicinal uses.
- Understanding the pharmacological properties of Z. mioga is crucial for validating its therapeutic potential.
- Previous research has not fully elucidated the neurobehavioral and electrophysiological effects of Z. mioga.
Purpose of the Study:
- To investigate the behavioral and electroencephalogram (EEG) effects of a water-soluble fraction of Z. mioga.
- To compare the effects of Z. mioga with known neuroleptic drugs like chlorpromazine and diazepam.
- To determine the potential neuroleptic-like activity of Z. mioga.
Main Methods:
- Administration of Z. mioga (50–400 mg/kg, i.p.) to mice and rats, and (20–30 mg/kg, i.v.) to rabbits.
- Behavioral assessments included open-field test, thiopental-induced sleeping time, conditioned avoidance response (shuttle box and step-down methods), and muscle relaxation.
- EEG recordings in rabbits monitored spontaneous activity, arousal responses to stimulation, and limbic after-discharges.
Main Results:
- Z. mioga significantly reduced locomotor activity, rectal temperature, and prolonged thiopental-induced sleep in mice.
- Z. mioga inhibited conditioned avoidance responses in rats but did not affect unconditioned escape responses.
- In rabbits, Z. mioga induced EEG patterns resembling drowsiness and inhibited arousal responses to external stimuli, similar to chlorpromazine.
Conclusions:
- The water-soluble fraction of Z. mioga exhibits significant neurobehavioral and EEG effects in animal models.
- The observed effects, particularly the reduction in locomotor activity and EEG changes, are qualitatively similar to those of neuroleptic drugs like chlorpromazine.
- These findings suggest that Z. mioga possesses neuroleptic-like properties, warranting further investigation into its active compounds and mechanisms of action.