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Studies on human urinary arylamidases.

P N Raina, S Ellis

    Archives Internationales De Physiologie Et De Biochimie
    |August 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Human urine contains enzymes that break down specific protein chains. Researchers studied their properties and found varying activity levels and sensitivities to inhibitors, providing insights into urinary enzyme function.

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    Area of Science:

    • Biochemistry
    • Urology

    Background:

    • Human urine contains various proteins, including enzymes with potential biological functions.
    • Understanding the enzymatic activity in urine can offer insights into physiological processes and potential biomarkers.

    Purpose of the Study:

    • To identify and characterize enzymes in human urine that hydrolyze specific arylamides.
    • To determine the kinetic properties, pH optima, and inhibitor profiles of these urinary arylamidases.

    Main Methods:

    • Enzyme assays were performed using leucyl-, alanyl-, and glycyl-prolyl-beta-naphthylamides as substrates.
    • Kinetic constants (Km) and pH optima were determined for each enzyme.
    • The effects of various inhibitors (Co2+, Mn2+, p-Chloromercuriphenyl-sulfonate, puromycin) were investigated.

    Main Results:

    • Human urinary protein contains enzymes that hydrolyze leucyl-, alanyl-, and glycyl-prolyl-beta-naphthylamides.
    • Kinetic constants (Km) were determined: 3.7 x 10(-5) M (leucyl), 7.1 x 10(-5) M (alanyl), and 1 x 10(-4) M (glycyl-prolyl).
    • pH optima were 7.4-7.8 for leucyl and alanyl arylamidases, and 8.8 for glycyl-prolyl arylamidase. Inhibitor studies revealed differential sensitivity.

    Conclusions:

    • Human urine possesses distinct arylamidase activities with characterized kinetic and chemical properties.
    • These enzymes exhibit varying responses to metal ions and chemical inhibitors, suggesting different structural or functional characteristics.
    • The study quantifies the 24-h urinary output of these enzymes in normal males, establishing baseline values.