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Related Experiment Video

Updated: Jul 25, 2026

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

Histone F1. Purification and phosphorus content.

R H Buckingham, L A Stocken

    The Biochemical Journal
    |March 1, 1970
    PubMed
    Summary

    Partial hepatectomy increases histone F1 phosphorylation. This suggests more histone F1 molecules are phosphorylated after liver regeneration, impacting cell division regulation.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cellular Biology

    Background:

    • Histone F1 is a key protein involved in DNA packaging and cell cycle regulation.
    • Liver regeneration, a complex process, involves significant cellular changes and protein modifications.

    Purpose of the Study:

    • To investigate the effect of partial hepatectomy on histone F1 phosphorylation.
    • To identify potential changes in histone F1 protein fractions during liver regeneration.

    Main Methods:

    • Separation of histone F1 protein fractions using DEAE-cellulose chromatography.
    • In vivo analysis of (32)P uptake into histone F1 and its fractions after partial hepatectomy.

    Main Results:

    • Two minor fractions, B and C, were isolated from histone F1, with fraction B being acidic.
    • Partial hepatectomy stimulated the in vivo uptake of (32)P into histone F1, but not into fractions B and C.
    • This indicates an increased rate of histone F1 phosphorylation in regenerating liver tissue.

    Conclusions:

    • Partial hepatectomy leads to an enhanced phosphorylation of histone F1.
    • The study suggests an increase in the total number of phosphorylated histone F1 molecules during liver regeneration.
    • These findings may have implications for understanding cell cycle control during tissue repair.

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