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Updated: Dec 11, 2025

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Published on: July 8, 2020

Cell proliferation in human melanoma.

S Shirakawa, J K Luce, I Tannock

The Journal of Clinical Investigation

|June 1, 1970

Summary

Melanoma metastases exhibit variable cell proliferation. Tumor cell growth fraction is 20-30%, with significant cell loss occurring, impacting tumor doubling time.

Area of Science:

Oncology
Cell Biology
Dermatology

Background:

Subcutaneous metastases in melanoma present a complex cellular dynamic.
Understanding tumor cell cytokinetics is crucial for predicting melanoma progression.

Purpose of the Study:

To investigate the cell cycle kinetics of subcutaneous melanoma metastases.
To determine the growth fraction and cell loss rate in these tumors.

Main Methods:

Utilized pulse labeling with tritiated thymidine in multiple biopsies.
Employed computer analysis for cell cycle phase duration (G2, S) and generation time (Te).
Assessed growth fraction using continuous/intermittent labeling and computer fitting.

Main Results:

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Identified a median G2 period of 5.3 hours and S period of 21 hours.
Determined a variable median generation time (Te) of 3 days.
Calculated a growth fraction of 20-30% and indicated approximately 70% cell loss.

Conclusions:

Melanoma metastases display significant heterogeneity in proliferation within tumors.
High cell loss rates contribute to a slower actual tumor doubling time than theoretically predicted.
These findings highlight the dynamic nature of melanoma metastasis and inform potential therapeutic strategies.