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Isolation of Leptospira of the serotype hardjo from bovine kidneys
Abstract:
Thirteen strains of Leptospira serotype hardjo were cultured from 200 bovine kidneys collected from an abattoir. All strains grew on primary isolation in EMJH medium 0.1 per cent Noble agar but most failed to grow in a variety of media containing neomycin or 5-fluorouracil as selective agents. Two of the cattle from which hardjo were isolated were seronegative by the microscopic agglutination test against hardjo, two had titres of 1/100, five of 1/400, three of 1/1600 and one of 1/6400.
Insights
Bovine kidneys yielded thirteen Leptospira serotype hardjo strains, challenging selective media. Most isolates grew on standard EMJH medium, indicating difficulties in selective isolation for bovine leptospirosis research.
Area of Science:
- Veterinary Microbiology
- Bacteriology
- Zoonotic Diseases
Background:
- Leptospira serotype hardjo is a significant cause of bovine abortion and infertility.
- Diagnosis often relies on serological methods, but culture is crucial for strain identification and characterization.
- Effective culture methods are essential for understanding Leptospira transmission dynamics in cattle.
Purpose of the Study:
- To isolate and identify Leptospira serotype hardjo from bovine kidneys.
- To evaluate the efficacy of different culture media for primary isolation.
- To correlate culture findings with serological titers in cattle.
Main Methods:
- Kidney samples were collected from 200 cattle at an abattoir.
- Primary isolation was attempted using EMJH medium with and without selective agents (neomycin, 5-fluorouracil).
- Microscopic agglutination test (MAT) was performed to determine antibody titers against Leptospira serotype hardjo.
Main Results:
- Thirteen strains of Leptospira serotype hardjo were successfully cultured from 200 bovine kidneys.
- All isolates grew on EMJH medium with 0.1% Noble agar.
- Most strains failed to grow on selective media containing neomycin or 5-fluorouracil.
- Serological titers in cattle ranged from seronegative to 1/6400.
Conclusions:
- Standard EMJH medium is effective for primary isolation of Leptospira serotype hardjo from bovine kidneys.
- Common selective agents may inhibit the growth of field strains of Leptospira serotype hardjo.
- Further optimization of selective culture media is needed for improved isolation rates.