[Effect of ampholines on blood coagulation: 1. Activation of factor VIII (antihemophilic globulin A)]

Polyaminopolycarboxyl acids (Ampholine, LKB producers Sweden) caused the antihaemophilic globulin (factor VIII) to be activated. This increase of activity revealed a linear dependence on Ampholine concentrations. Only those Ampholine molecules of alkaline pH areas positively charged under test conditions turned out to be efficient. The positive charge, therefore, seems to be significant for an activation. The coagulation activities were measured by determining the partial thromboplastin time (PTT) and the factor VIII in an one-phase test.