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Related Experiment Videos

Adenosine triphosphate pools in Methanobacterium.

A M Roberton, R S Wolfe

    Journal of Bacteriology
    |April 1, 1970
    PubMed
    Summary
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    Adenosine triphosphate (ATP) metabolism in Methanobacterium is inefficient, with low ATP yield during growth. Several compounds disrupt ATP levels, suggesting they inhibit electron transport or uncouple energy processes in this strict anaerobe.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Energy Metabolism

    Background:

    • Adenosine triphosphate (ATP) is the primary energy currency in cells.
    • Methanobacterium is a strict anaerobe crucial in methane production.
    • Understanding ATP metabolism is key to microbial energy conservation.

    Purpose of the Study:

    • To investigate ATP metabolism in Methanobacterium strain M.o.H.
    • To determine ATP conservation efficiency during bacterial growth.
    • To identify factors affecting ATP levels and methane formation.

    Main Methods:

    • Growth yield studies in a fermentor.
    • Cell-free extract experiments for ATP formation.
    • Whole-cell studies measuring ATP, ADP, and AMP pools.

    Related Experiment Videos

  • Development of a rapid anaerobic cell sampling apparatus.

    • Main Results:

    • Low ATP conservation efficiency (0.06 mol ATP/mol H2) observed.
    • Net ATP formation was not detected in cell-free extracts.
    • Air, chloroform, and phenols decreased ATP and increased AMP in whole cells.
    • These compounds inhibited methane formation in cell-free extracts.

    Conclusions:

    • ATP conservation is inefficient in Methanobacterium under tested conditions.
    • Investigated compounds likely act as electron transport inhibitors or uncouplers.
    • Methane formation, an ATP-dependent process, is inhibited by these compounds.