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An artificial leucocyte control suspension.

T R Lappin, F M Sanderson

    Journal of Clinical Pathology
    |February 1, 1970
    PubMed
    Summary
    This summary is machine-generated.

    Candida albicans yeast cells offer a stable, easily prepared alternative to leucocytes for laboratory use. This yeast suspension mimics leucocyte size and withstands conditions causing red blood cell lysis.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Biotechnology

    Background:

    • Leucocyte suspensions are crucial for various biological assays.
    • Challenges exist in preparing and maintaining stable leucocyte controls.
    • Alternative cell models are needed for consistent experimental outcomes.

    Purpose of the Study:

    • To evaluate Candida albicans yeast as a viable leucocyte control.
    • To assess the suitability of yeast cells for biological assays requiring leucocyte mimics.
    • To establish a stable and easily prepared alternative to traditional leucocyte controls.

    Main Methods:

    • Culturing Candida albicans under specific environmental conditions to obtain the yeast form.
    • Preparing a stable suspension of yeast cells.

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  • Evaluating yeast cell size and uniformity relative to leucocytes.
  • Testing the resilience of yeast cell suspensions under conditions causing red blood cell lysis.

    • Main Results:

    • Candida albicans yeast cells can be reliably cultured to yield a uniform suspension.
    • Yeast cells exhibit comparable size to leucocytes.
    • The yeast suspension remains stable under conditions that induce lysis in red blood cells.
    • The preparation method is straightforward and scalable for large quantities.

    Conclusions:

    • Candida albicans yeast provides a practical and effective substitute for leucocyte controls in laboratory settings.
    • The stability and ease of preparation make this yeast suspension a valuable tool for research.
    • This method offers a consistent and reliable alternative for assays dependent on leucocyte mimics.