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Growth of Histoplasma capsulatum. VI. Maintenance of the mycelial phase

L Pine

    Applied Microbiology
    |March 1, 1970
    PubMed

    Insights

    This study details a new medium for maintaining Histoplasma capsulatum's original sporulation. A yeast phase medium also indirectly preserves the mycelial characteristics of this fungus.

    Area of Science:

    • Medical Mycology
    • Microbiology

    Background:

    • Histoplasma capsulatum exists in mycelial and yeast phases.
    • Maintaining the original characteristics of these phases is crucial for research.

    Purpose of the Study:

    • To describe a novel medium for preserving the sporulating mycelial phase of Histoplasma capsulatum.
    • To re-evaluate a yeast phase medium for its efficacy in supporting the yeast form and indirectly maintaining mycelial characteristics.

    Main Methods:

    • Cultivation of Histoplasma capsulatum on a newly described medium.
    • Utilizing a specific yeast phase medium for conversion and maintenance.

    Main Results:

    • The described medium successfully maintains the mycelial phase in its original sporulating condition.
    • The yeast phase medium effectively supports the yeast form and indirectly preserves original mycelial traits.

    Conclusions:

    • A specialized medium is effective for maintaining Histoplasma capsulatum mycelial sporulation.
    • Yeast phase medium serves a dual role in supporting yeast growth and indirectly preserving mycelial characteristics.

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