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Acetabularia chloroplast DNA: electron microscopic visualization.

B R Green, H Burton

    Science (New York, N.Y.)
    |May 22, 1970
    PubMed
    Summary
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    Isolated chloroplasts released large amounts of supercoiled DNA, potentially similar to bacterial DNA levels. This DNA was observed in organized loops, suggesting a unique structural arrangement within the chloroplasts.

    Area of Science:

    • * Cell Biology
    • * Molecular Biology
    • * Genetics

    Background:

    • * Chloroplasts, the sites of photosynthesis in eukaryotic cells, contain their own genetic material.
    • * Understanding the structure and organization of chloroplast DNA is crucial for comprehending chloroplast function and evolution.

    Purpose of the Study:

    • * To investigate the DNA content and organization within isolated Acetabularia mediterranea chloroplasts.
    • * To characterize the physical state and structural arrangement of chloroplast DNA.

    Main Methods:

    • * Isolation of chloroplasts from Acetabularia mediterranea.
    • * Lysis of chloroplasts via osmotic shock in a protein monolayer.
    • * Microscopic observation of released DNA structures.

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    Main Results:

    • * Significant quantities of supercoiled DNA were released from lysed chloroplasts.
    • * The released DNA often formed large loops originating from organized centers.
    • * The amount of DNA per chloroplast was comparable to that found in bacteria.

    Conclusions:

    • * Acetabularia mediterranea chloroplasts contain substantial amounts of supercoiled DNA.
    • * Chloroplast DNA exhibits organized looping structures, indicating specific packaging mechanisms.
    • * The DNA content suggests a significant genetic capacity within these organelles.