Circadian rhythm of brain self-stimulation behavior

Under constant conditions of light, sound, temperature, and humidity, rats exhibited circadian rhythmicity in rate of bar-pressing with hypothalamic and septal reinforcing brain stimulation. Variations in reinforcer magnitude aflected absolute levels of operant response emission but not the frequency of the circadian rhythm. In long sessions, the time of peak responding deviated systematically from a strict 24-hour period. Such data show marked similarity to free-running rhythms of motor activity.