Related Experiment Video
Updated: Aug 10, 2026

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
Published on: March 29, 2010
Assay of anticomplementary activity in solutions of immunoglobulins
Abstract:
A method of estimating the inactivation of complement by immunoglobulins, based on a hemolytic assay, is described. During a three year period of routine assays the investigation of standard immunoglobulin solutions of high and low anticomplementary activity confirmed the reliability of the method. Since the activity of complement is largely dependent on the ionic strength, the salt concentration of the test solution must be carefully adjusted. The specific complement inactivation per gram immunoglobulin CI50/g is proposed as a parameter to compare the anticomplementary activity of immunoglobulin solutions.
More Related Videos
06:29Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
06:15Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
Published on: September 7, 2018
Related Concept Videos
Immunoprecipitation
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Antibody Actions
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...