Improved methodology for the quantitative analysis of mixtures by band centrifugation: reversible equilibration in...

Double-sector aluminium centerpieces are modified for use in analytical high-speed band centrifugation up to 60,000 rpm. A method has been developed for testing IG preparations according to administrative regulations with special regard to precision and reproducibility. The proposed band-forming centrifugation method with scanner registration in UV absorption optics and special evaluation of the curves gives values in comparison to well-known procedures by Svedberg and Pedersen. Subjective and systematic errors are especially estimated with this method and the sensitivity is best suited to automatic computerization and results are therefore readily available at the end of the centrifuge run within a few minutes. Investigations have been undertaken to analyze reversible equilibration in immunoglobulin preparations; the results are discussed.