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Related Experiment Videos

Indirect visualization of Staphylococcus aureus protein A.

D S Nickerson, J G White, G Kronvali

    The Journal of Experimental Medicine
    |May 1, 1970
    PubMed
    Summary

    Staphylococcal protein A, found on the bacterial cell wall, binds to gamma globulin. This interaction may trigger immune responses like inflammation and pus formation even without prior immunity.

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    Area of Science:

    • Microbiology
    • Immunology
    • Cell Biology

    Background:

    • Staphylococcal protein A is known to interact with the Fc portion of gamma globulin.
    • The precise location and role of protein A in host interactions are not fully understood.

    Purpose of the Study:

    • To ultrastructurally localize staphylococcal protein A on the bacterial cell surface.
    • To investigate the implications of protein A's location for host-pathogen interactions.

    Main Methods:

    • Ultrastructural studies using intact staphylococci.
    • Labeling of myeloma gamma G-globulin to detect protein A binding.

    Main Results:

    • Protein A was localized to the outermost layer of the staphylococcal cell wall.
    • Strains expressing protein A showed a coating of gamma globulin on their surface, unlike strains lacking protein A.

    Conclusions:

    • Staphylococcal protein A is exposed on the bacterial surface, representing the initial point of contact with the host.
    • Protein A-immunoglobulin interactions can initiate complement activation and inflammatory responses, potentially explaining cross-reactivity and pathogenicity in non-immune hosts.

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