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Genetic transmission of Moloney leukemia virus: mapping of the chromosomal integration site
Abstract:
Mice genetically transmitting the exogenous Moloney leukemia virus (Balb/Mo) have been previously derived. These animals carried one copy of Moloney virus DNA (M-MuLV) in their germ line and transmitted the virus as a single Mendelian gene to the next generation. Homozygous BALB/Mo mice were used to genetically map the M-MuLV locus. Embryo fibroblasts were fused to established Chinese hamster cells and somatic cell hybrids were selected. Segregation of mouse chromosomal markers in the hybrids was correlated to the loss of M-MuLV-specific sequences as detected by molecular hybridization. Of 15 isozymes located on different mouse chromosomes only triosephosphate isomerase segregated syntenic with the M-MuLV gene, suggesting that the virus was integrated on chromosome No. 6. This was confirmed by sexual genetic experiments analyzing segregation of Moloney viremia and two markers on chromosome 6 and 15, respectively. The results show that M-MuLV expression is linked to wa-1 on chromosome 6 at a distance of about 30 map units. These data define a new genetic locus, Mov-1, representing the structural gene of M-MuLV in BALB/Mo mice.
Insights
Researchers mapped the Moloney leukemia virus (M-MuLV) genetic locus in BALB/Mo mice. They identified the virus
Area of Science:
- Genetics
- Virology
- Molecular Biology
Background:
- BALB/Mo mice carry exogenous Moloney leukemia virus (M-MuLV) in their germ line.
- The M-MuLV is transmitted as a single Mendelian gene.
- Previous studies established the genetic basis of M-MuLV transmission in these mice.
Purpose of the Study:
- To genetically map the M-MuLV locus in BALB/Mo mice.
- To identify the specific chromosome and location of the M-MuLV integration.
- To define a new genetic locus for M-MuLV.
Main Methods:
- Somatic cell hybridization of mouse embryo fibroblasts with Chinese hamster cells.
- Selection of somatic cell hybrids and analysis of segregated mouse chromosomal markers.
- Molecular hybridization to detect M-MuLV-specific sequences.
- Sexual genetic experiments analyzing viremia and chromosome-specific markers.
Main Results:
- Triosephosphate isomerase was the only isozyme that segregated syntenically with the M-MuLV gene among 15 tested.
- The M-MuLV gene was localized to mouse chromosome 6.
- M-MuLV expression was found to be linked to wa-1 on chromosome 6, approximately 30 map units away.
Conclusions:
- The M-MuLV integration site in BALB/Mo mice is on chromosome 6.
- A new genetic locus, Mov-1, representing the structural gene of M-MuLV, has been defined.
- This study provides precise genetic mapping of the M-MuLV locus in BALB/Mo mice.