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Cyclic cytidine 2',3'-phosphate: molecular structure.

C L Coulter, M L Greaves

    Science (New York, N.Y.)
    |September 11, 1970
    PubMed
    Summary
    This summary is machine-generated.

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    This study details the crystal structure of cytidine 2',3'-phosphate, revealing antiparallel nucleotide stacking and specific base and sugar conformations. These findings offer insights into nucleic acid structural dynamics.

    Area of Science:

    • Biochemistry
    • Crystallography
    • Molecular Biology

    Background:

    • Cytidine 2",3"-phosphate is a cyclic nucleotide with biological relevance.
    • Understanding nucleotide structure is crucial for molecular biology and drug design.

    Purpose of the Study:

    • To elucidate the three-dimensional structure of the sodium salt of cytidine 2",3"-phosphate at the atomic level.
    • To characterize the conformation of the base, ribose sugar, and phosphate groups within the crystal lattice.

    Main Methods:

    • Single-crystal X-ray diffraction analysis was performed on monoclinic crystals.
    • The crystal structure was solved and refined to determine atomic positions and bonding.

    Main Results:

    • The asymmetric unit contained two cytidine 2",3"-phosphate anions.

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  • Both bases adopted a syn conformation, forming an antiparallel stranded ribbon with bases 3.3 Å apart.
  • One ribose ring was planar, while the other showed puckering; phosphorus atoms were puckered towards the sugars.
  • Specific conformations (gauche-trans, gauche-gauche) were observed around the C4"-C5" bonds.

    • Conclusions:

    • The study provides a detailed structural model for cytidine 2",3"-phosphate.
    • The observed conformations highlight the flexibility and specific structural preferences of nucleotides.
    • This structural information can inform studies on nucleic acid interactions and enzymatic mechanisms.