Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoglobulin elution from multiple sclerosis brain.

D Gilden, T Tachovsky

    Journal of Neuroscience Methods
    |August 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Varicella zoster virus vasculopathy: analysis of virus-infected arteries.

    Neurology·2011
    Same author

    Analysis of breast-tissue cathepsin-d isoforms from patients with breast-cancer, benign breast disease and from normal controls.

    International journal of oncology·2011
    Same author

    Review: The neurobiology of varicella zoster virus infection.

    Neuropathology and applied neurobiology·2011
    Same author

    Efficacy of live zoster vaccine in preventing zoster and postherpetic neuralgia.

    Journal of internal medicine·2011
    Same author

    Zoster sine herpete: virologic verification by detection of anti-VZV IgG antibody in CSF.

    Neurology·2011
    Same author

    Absence of Epstein-Barr virus in the brain and CSF of patients with multiple sclerosis.

    Neurology·2010
    Same journal

    Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

    Journal of neuroscience methods·2026
    Same journal

    Assessment metrics for pain control in rats: A methodological commentary.

    Journal of neuroscience methods·2026
    Same journal

    Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

    Journal of neuroscience methods·2026
    Same journal

    Methods for measuring neural activity during voluntary wheel running.

    Journal of neuroscience methods·2026
    Same journal

    Serotype-dependent differences in AAV cellular transduction rates in the hypothalamus of Arctic ground squirrels.

    Journal of neuroscience methods·2026
    Same journal

    Rapid generation of human sensory neurons from iPSC for modeling of peripheral neuropathies.

    Journal of neuroscience methods·2026
    See all related articles

    Immunoglobulin (Ig) was successfully isolated from multiple sclerosis (MS) brain tissue. This method allows for better characterization of Ig in MS brains compared to control samples.

    Area of Science:

    • Neuroimmunology
    • Biochemistry
    • Pathology

    Background:

    • Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system.
    • The role of immunoglobulin (Ig) deposition in MS pathogenesis is not fully understood.
    • Previous methods for isolating Ig from brain tissue have limitations.

    Purpose of the Study:

    • To develop and validate a method for efficient elution and isolation of immunoglobulin from multiple sclerosis (MS) brain tissue.
    • To compare Ig levels in MS brain tissue with non-MS control brain tissue.
    • To facilitate the characterization of cell-surface Ig in MS.

    Main Methods:

    • White matter slices from MS and control brains were washed with phosphate-buffered saline (PBS).
    • Tissue was treated with acetic acid (pH 2.5) containing protease inhibitors for Ig elution.

    Related Experiment Videos

  • Protein concentration and Ig levels in eluates were determined using competitive microradioimmunoassay.
  • Ig was characterized using DEAE cellulose column chromatography.

    • Main Results:

    • Successive PBS washes effectively removed extracellular proteins.
    • Equivalent protein quantities were recovered from MS and control brain samples after elution.
    • Significantly less protein was required for 50% inhibition of [125I]IgG binding in MS brain eluates compared to controls (P < 0.05).
    • Isolated Ig eluted in the void volume of a DEAE cellulose column, indicating its large molecular size.

    Conclusions:

    • The described technique effectively isolates immunoglobulin from MS brain tissue.
    • The findings suggest increased or altered Ig deposition in MS brain.
    • This method provides a valuable tool for studying cell-surface Ig in MS pathogenesis.