Hypothalamic uptake of tritiated testosterone in the sheep following intracerebroventricular infusion

A method is described for the intracerebroventricular infusion of labelled testosterone in conscious sheep. Our objective was to establish whether the infused testosterone entered the nuclei of cells in the hypothalamic region. Since the results from castrated males showed testosterone to be taken up by cell nuclei, in the hypothalamic region, the method promises to be useful for the study of the hypothalamic metabolism of androgens in relation to the sexual behaviour and neuroendocrine function in large animals.