Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gentamicin resistance in Staphylococcus aureus.

A Porthouse, D F Brown, R G Smith

    Lancet (London, England)
    |January 3, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sabulodes caberata guenée (Lepidoptera: Geometridae) : Components of its sex pheromone gland.

    Journal of chemical ecology·2013
    Same author

    Insect sex pheromones : Effect of temperature on evaporation rates of acetates from rubber septa.

    Journal of chemical ecology·2013
    Same author

    Mint root borer,Fumibotys fumalis (Guenee): Identification and field tests of female sex pheromone gland components.

    Journal of chemical ecology·2013
    Same author

    X-ray-induced mutations in extracellular phage.

    Nature·2010
    Same author

    Two Structurally Distinct VanA Resistance Elements at Different Locations in a Glycopeptide-Resistant Strain of Enterococcus faecalis.

    Antimicrobial agents and chemotherapy·2006
    Same author

    Treatment options for deep venous thrombosis.

    Emergency medicine clinics of North America·2002

    This study identified a Staphylococcus aureus strain producing an enzyme that inactivates common antibiotics like gentamicin. Researchers are investigating the enzyme

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Antimicrobial Resistance

    Background:

    • Clinical isolates of Staphylococcus aureus can exhibit resistance to multiple antibiotics.
    • Aminoglycoside antibiotics (gentamicin, kanamycin, tobramycin) are crucial for treating S. aureus infections.
    • Enzyme-mediated drug inactivation is a common mechanism of antibiotic resistance.

    Purpose of the Study:

    • To characterize the antibiotic resistance mechanism in a clinical isolate of Staphylococcus aureus.
    • To investigate the genetic basis for the production of a drug-inactivating enzyme.
    • To determine the cellular location of the genetic determinants responsible for enzyme synthesis.

    Main Methods:

    • Phenotypic characterization of antibiotic resistance in Staphylococcus aureus.

    Related Experiment Videos

  • Enzyme assays to detect drug-inactivating activity.
  • Molecular techniques to investigate the genetic determinants of enzyme production (e.g., plasmid analysis, gene sequencing - specific methods not detailed in abstract).

    • Main Results:

    • The clinical isolate of Staphylococcus aureus demonstrated resistance to gentamicin, kanamycin, and tobramycin.
    • The resistance was attributed to the production of a specific drug-inactivating enzyme.
    • Attempts to localize the genetic determinants for enzyme synthesis yielded inconclusive results.

    Conclusions:

    • A clinical Staphylococcus aureus isolate possesses a novel aminoglycoside-inactivating enzyme.
    • The genetic location of the enzyme's determinants remains undetermined, requiring further investigation.
    • Understanding this resistance mechanism is vital for developing strategies against multidrug-resistant bacteria.