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Amphotericin B serum concentrations during therapy.

B T Fields, J H Bates, R S Abernathy

    Applied Microbiology
    |June 1, 1970
    PubMed
    Summary
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    Accurate measurement of amphotericin B serum concentrations is crucial for treating systemic mycoses. This study refined drug level analysis, revealing previously underestimated therapeutic drug concentrations.

    Area of Science:

    • Pharmacology
    • Mycology
    • Clinical Chemistry

    Background:

    • Therapeutic outcomes in systemic mycoses correlate with amphotericin B serum concentrations.
    • Existing data on amphotericin B serum levels are conflicting, necessitating re-evaluation.

    Purpose of the Study:

    • To accurately determine amphotericin B serum concentrations in patients undergoing treatment for systemic mycoses.
    • To clarify the relationship between drug levels and therapeutic efficacy.

    Main Methods:

    • Utilized a precise and accurate bioassay for quantifying serum amphotericin B levels.
    • Measured drug concentrations at multiple time points post-intravenous infusion (1, 18, and 42 hours).
    • Assessed drug persistence in serum up to 13 weeks post-treatment and stability in stored samples.

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    Main Results:

    • Highest measured serum concentration was 2.01 mug/ml.
    • Mean concentrations at 1, 18, and 42 hours were 1.21, 0.62, and 0.32 mug/ml, respectively.
    • Amphotericin B was detectable up to 7 weeks post-treatment; levels remained stable in serum stored for 8-9 months.

    Conclusions:

    • The study provides precise amphotericin B serum concentration data, potentially explaining previous discrepancies.
    • Accurate bioassays are essential for reliable therapeutic drug monitoring in systemic fungal infections.
    • Refined understanding of amphotericin B pharmacokinetics aids in optimizing treatment strategies.