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The interaction of monosodium urate with connective tissue components
The Journal of Clinical Investigation
|October 1, 1970
Summary
Cartilage components called proteinpolysaccharides (PPL) significantly increase urate solubility, preventing crystal formation in gout patients. Their molecular integrity is crucial for this effect.
Area of Science:
- Biochemistry
- Connective Tissue Research
- Gout Pathophysiology
Background:
- Monosodium urate crystals are the primary cause of gout, depositing mainly in connective tissues.
- The solubility of urate in bodily fluids is a key factor in preventing crystal deposition.
Purpose of the Study:
- To investigate the effect of bovine nasal cartilage components on urate solubility.
- To identify the specific cartilage components responsible for enhancing urate solubility and preventing urate crystallization.
Main Methods:
- Preparation of acetone-dried homogenates from bovine nasal cartilage.
- Testing the effect of cartilage homogenates and their components on urate solubility in buffered solutions.
- Assessing the impact of proteinpolysaccharide (PPL) concentration and molecular integrity on urate solubility.
Main Results:
- Bovine nasal cartilage homogenates significantly enhanced urate solubility.
- Proteinpolysaccharides (PPL), compounds of protein and chondroitin sulfate, were identified as the active components.
- Increased PPL concentration correlated with increased urate solubility, and molecular integrity was essential.
- Specific PPL subfractions, PPL(5) and PPL(3), showed differential effects on urate solubility.
- PPL molecules also inhibited urate crystallization from supersaturated solutions.
Conclusions:
- Proteinpolysaccharides in cartilage play a crucial role in maintaining urate solubility.
- The enzymatic destruction of PPL during connective tissue turnover may lead to urate crystal precipitation in gout.
- Further research is needed to elucidate the exact mechanism of urate solubilization by PPL.
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