Total nitrogen and L-hydroxyproline content of adipose tissue from various sites in rats of different ages

The total nitrogen and L-hydroxyproline concentrations of two intra-abdominal and two subcutaneous sites of adipose tissue were measured in male and female Wistar rats. At 100 g body weight the nitrogen concentration of intra-abdominal adipose tissue was slightly lower than that of subcutaneous adipose tissue. As age advanced beyond sexual maturity, the nitrogen content of intra-abdominal adipose tissue was steadily reduced, whereas that of subcutaneous tissue did not change. The L-hydroxyproline concentration of intra-abdominal adipose tissue was also lower than in subcutaneous adipose tissue. In male rats it rose in most adipose tissue sites when sexual maturity was reached, then fell with advancing age. In female rats the pattern of change was less consistent, but the l-hydroxyproline content of intra-abdominal adipose tissue was reduced as age increased.