[Some specific features of enzymatic dehydration of tetrahydro-1,4-benzdiazepine-2-one]

Differences in the dehydration rates of tetrahydro-1,4-benzdiazepine-2-one in the liver, kidney and adrenals of mice, rats and guinea pigs have been revealed. It has been found that the enzyme catalyzing the substrate conversion into its dihydroderivative is localized in the microsomal and soluble fractions of the organs tested and has a pH optimum of 9,0. The enzyme activity depends on the concentation of phosphate ions in the incubation medium.