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Chlorine dioxide metabolism in rat.
Journal of Environmental Pathology and Toxicology
|December 1, 1979
Summary
This study developed a new assay to measure chlorine dioxide (ClO2) metabolites in biological fluids. Results show Cl-, ClO-2, and ClO-b are key metabolites, with varying plasma absorption based on dosage.
Area of Science:
- Environmental Science
- Toxicology
- Analytical Chemistry
Background:
- Chlorine dioxide (ClO2) is a potential alternative disinfectant for public water supplies.
- Understanding ClO2 metabolism is crucial for assessing its safety and efficacy.
Purpose of the Study:
- To develop a novel assay for detecting and quantifying 36ClO2 metabolites.
- To investigate the metabolic fate of ClO2 in biological systems, specifically in rats.
Main Methods:
- Development of a new qualitative and quantitative assay for 36ClO2 metabolites.
- Administration of 36ClO2 to rats via oral doses.
- Analysis of metabolites in water and biological fluids (plasma, packed cells).
Main Results:
- Identified chloride (Cl-), chlorite (ClO-2), and chlorobite (ClO-b) as primary metabolites of ClO2.
- Observed higher percentage of ClO; formed in plasma from a 10 mg/l dose compared to a 100 mg/l dose.
- Demonstrated that 36Cl readily exchanges with chloride in saline, as indicated by packed cell concentrations.
Conclusions:
- The developed assay effectively measures ClO2 metabolites.
- The metabolic profile of ClO2 includes Cl-, ClO-2, and ClO-b.
- Dosage influences the formation of ClO; in plasma, suggesting complex pharmacokinetic behavior.