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Molecular weight of Sindbis virus ribonucleic acid as measured by polyacrylamide gel electrophoresis

P Dobos, P Faulkner

    Journal of Virology
    |July 1, 1970
    PubMed

    Insights

    The molecular weight of Sindbis virion RNA was determined using gel electrophoresis. Upon denaturation, it yielded fragments of approximately 1.76 x 10(6) daltons, indicating the size of Sindbis 26S RNA.

    Area of Science:

    • Molecular Biology
    • Virology

    Background:

    • Sindbis virus is a well-studied alphavirus.
    • Understanding viral RNA molecular weight is crucial for molecular biology and virology research.

    Purpose of the Study:

    • To determine the molecular weight of Sindbis virion ribonucleic acid (RNA).
    • To analyze the fragmentation pattern of Sindbis RNA upon denaturation.

    Main Methods:

    • Gel electrophoresis was used to determine the molecular weight of Sindbis virion RNA.
    • Urea denaturation was employed to analyze RNA fragmentation.

    Main Results:

    • The molecular weight of Sindbis virion RNA ranged from 3.89 x 10(6) to 4.45 x 10(6) daltons.
    • Denaturation with urea resulted in fragments with a molecular weight of 1.76 x 10(6) daltons.
    • Viral-specific Sindbis 26S RNA was identified as a major species with a molecular weight of 1.76 x 10(6) daltons.

    Conclusions:

    • The study precisely quantified the molecular weight of Sindbis virion RNA.
    • The findings confirm the size of the major Sindbis 26S RNA species.

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