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Updated: Aug 6, 2026

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Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
Published on: March 28, 2011
Electron probe x-ray microanalysis of a normal centriole
Abstract:
A scanning spectrometer with lithium fluoride, ammonium dihydrogen phosphate, and gypsum crystals was used to detect the x-ray spectrum of a normal centriole, the transmitted electron image of which was used to focus the exciting electron beam to the size, shape, and position of the centriole in a 700-angstrom section of intact tissue.
Related Concept Videos
Scanning Electron Microscopy
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Fundamental Principles
Accelerated...
Electron Microscope Tomography and Single-particle Reconstruction
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Centrioles and Centrosomes
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Near the end of the prophase, also called late prophase or "prometaphase,"...

