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Laboratory studies with a selective Enterococcus medium.

H D Isenberg, D Goldberg, J Sampson

    Applied Microbiology
    |September 1, 1970
    PubMed
    Summary
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    A new Selective Enterococcus agar effectively identifies Lancefield group D streptococci, commonly known as enterococci, in clinical samples. This medium offers superior selectivity compared to existing options, aiding in the presumptive recognition of these infectious agents.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Bacteriology

    Background:

    • Lancefield group D streptococci, or enterococci, are frequently implicated in infectious diseases.
    • Accurate and timely presumptive identification of enterococci in clinical specimens is challenging with current laboratory media.
    • Selective Enterococcus agar was developed to improve the isolation of enterococci.

    Purpose of the Study:

    • To evaluate the efficacy of a new Selective Enterococcus agar for the presumptive identification of enterococci.
    • To assess the selectivity and sensitivity of Selective Enterococcus agar compared to established culture media.
    • To determine the colonial morphology of enterococci on the new medium.

    Main Methods:

    • Selective Enterococcus agar was tested using clinical specimens containing various microorganisms.

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  • The performance of Selective Enterococcus agar was compared against Trypticase Soy agar with sheep blood and Mitis Salivarius agar.
  • Enterococci isolates were biochemically and immunochemically classified according to standard criteria.

    • Main Results:

    • Selective Enterococcus agar displayed characteristic black zones around enterococcal colonies.
    • Listeria monocytogenes showed similar colonial morphology after 48 hours; most other bacteria were inhibited or showed different morphology.
    • The sensitivity of Selective Enterococcus agar was comparable to blood and Mitis-Salivarius agars, but its selectivity was superior.

    Conclusions:

    • Selective Enterococcus agar provides a highly selective method for the presumptive identification of enterococci in clinical settings.
    • The medium's distinct colonial appearance aids in differentiating enterococci from other bacteria, including Listeria monocytogenes.
    • This new agar represents a valuable improvement for clinical laboratories dealing with enterococcal infections.