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Related Experiment Videos

Folate binders in body fluids.

F P Retief, Y J Huskisson

    Journal of Clinical Pathology
    |November 1, 1970
    PubMed
    Summary
    This summary is machine-generated.

    Saline dialysis and charcoal adsorption effectively clear folate from serum, with variations observed in kwashiorkor. Folate exists in both free and bound forms in various bodily fluids, with distinct profiles in different physiological states.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry

    Background:

    • Folate is essential for numerous metabolic processes.
    • Understanding folate binding and clearance is crucial for nutritional and clinical assessments.

    Purpose of the Study:

    • To compare folate clearance using saline dialysis and haemoglobin-coated charcoal adsorption.
    • To investigate the forms of folate in various biological fluids and their dialyzability.
    • To characterize the predominant serum folate binder.

    Main Methods:

    • Saline dialysis of serum using Visking casing.
    • Haemoglobin-coated charcoal adsorption.
    • Chromatography on Sephadex G-25.
    • Adsorption studies with molecular sieves.

    Main Results:

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    • Saline dialysis and charcoal adsorption showed comparable folate clearance, except in kwashiorkor where charcoal was more effective.
    • Urinary folate was primarily dialyzable (free).
    • Saliva, bile, and erythrocytes contained both dialyzable and non-dialyzable folate fractions.
    • Pregnancy urine showed increased total folate, while nephrotic urine did not.
    • Serum folate chromatography revealed two peaks, one associated with proteins; urine showed only the second peak.
    • The primary serum folate binder has a molecular weight of 70,000-120,000 and is likely not albumin.

    Conclusions:

    • Saline dialysis is a viable method for folate clearance, comparable to charcoal adsorption in normal states.
    • Folate exists in different forms (free and bound) across various bodily fluids, with altered proportions in conditions like kwashiorkor and pregnancy.
    • The main serum folate binding protein is a distinct entity, likely not albumin, with a significant molecular weight.