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Related Experiment Videos

Surface ultrastructure of human skin.

K Hashimoto, T Kanzaki

    Acta Dermato-Venereologica
    |January 1, 1975
    PubMed
    Summary

    Human skin

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    Area of Science:

    • Dermatology
    • Cell Biology
    • Microscopy

    Background:

    • The human skin surface's intricate structure is crucial for its barrier function.
    • Understanding the topographical features of the outermost skin layer (stratum corneum) is essential for dermatological research.

    Purpose of the Study:

    • To elucidate the detailed surface morphology of normal human skin using advanced microscopy techniques.
    • To investigate the arrangement and characteristics of horny cells and their interactions.

    Main Methods:

    • Utilized a combination of scanning electron microscopy (SEM), surface replication, and transmission electron microscopy (TEM).
    • Employed critical point drying (CPD) for specimen preparation to preserve delicate structures.
    • Examined both native human skin and tissue-cultured keratinocytes.

    Main Results:

    • Observed horny cells (corneocytes) forming columnar stacks with overlapping peripheries.
    • Identified distinct surface features on corneocytes, including central dome-shaped elevations and peripheral imprints from overlapping cells.
    • Revealed villi on the underside of corneocytes, except at overlapping zones.
    • Tissue-cultured keratinocytes exhibited similar stacking and overlapping, but without imprints, suggesting a lack of pressure.

    Conclusions:

    • The study provides a high-resolution ultrastructural description of the human skin surface.
    • The findings clarify the cellular arrangement and surface topography of the stratum corneum.
    • The comparison with cultured keratinocytes offers insights into the role of pressure in forming surface imprints.

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