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Updated: Aug 15, 2026

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Published on: May 6, 2010
Scanning electron microscopy-aided observations on and therapy of teat canal infections
Abstract:
An examination of teat canal swabs established that 51 teat canals out of 68 quarters of machine-milked cows were colonized by Staphylococcus aureus. Only 31 of these quarters yielded milk from which S. aureus could be cultured, and 6 out of the 31 produced milk containing somatic cell counts in excess of 500 000/ml. No inhibitory substances could be detected in milk samples 12 h after 10 mg of sodium cloxacillin had been deposited in the test canal on 1-4 successive occasions. Teat canal swabs and milk sample cultures of the same quarters became and remained bacteriologically negative for at least a week after the last treatment. Six quarters, which according to the International Dairy Federation criteria were suffering from subclinical mastitis, became negative after local teat canal therapy. Scanning electron micrographs of one infected teat canal revealed the presence of cocci in depressions and crevices on the epithelial surface, suggesting that such cocci are not always flushed out into milk samples. Teat canal therapy should make a marked contribution to the control of bovine mastitis.
Insights
Local teat canal therapy effectively eliminated Staphylococcus aureus in machine-milked cows. This treatment reduced bacterial colonization and resolved subclinical mastitis, offering a promising strategy for bovine mastitis control.
Area of Science:
- Veterinary Medicine
- Microbiology
- Animal Science
Background:
- Bovine mastitis is a significant economic concern in dairy farming.
- Staphylococcus aureus is a primary pathogen causing mastitis.
- Effective treatment strategies for bovine mastitis are crucial for herd health.
Purpose of the Study:
- To evaluate the efficacy of local teat canal therapy for Staphylococcus aureus colonization in machine-milked cows.
- To assess the impact of teat canal therapy on subclinical mastitis.
- To investigate the presence of Staphylococcus aureus within teat canal structures.
Main Methods:
- Sampling of teat canals and milk from machine-milked cows.
- Bacteriological culture for Staphylococcus aureus detection.
- Somatic cell count analysis of milk samples.
- Administration of sodium cloxacillin via teat canal therapy.
- Scanning electron microscopy of teat canal tissues.
Main Results:
- Staphylococcus aureus colonized 51 out of 68 teat canals.
- Only 31 colonized quarters yielded S. aureus in milk cultures.
- Six quarters with subclinical mastitis resolved after teat canal therapy.
- Bacteriological cultures were negative for at least one week post-treatment.
- Microscopy revealed cocci within teat canal epithelial crevices.
Conclusions:
- Local teat canal therapy is effective in eradicating Staphylococcus aureus from infected quarters.
- This therapeutic approach can successfully treat subclinical bovine mastitis.
- Teat canal therapy represents a valuable tool for controlling bovine mastitis.
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