Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 17, 2026

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Published on: April 10, 2016

Rapid assay for bactericidal antibodies.

G D Hansen, A S Armstrong

Applied Microbiology

|August 1, 1970

Summary

Microtiter plates can replace tubes for bactericidal tests of opsonic antibodies. This efficient method is ideal for screening numerous serum samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Single dose streptozotocin-induced diabetes: considerations for study design in islet transplantation models.

Laboratory animals·2011

Same author

Malthus to Solow.

Working paper series (National Bureau of Economic Research)·2002

Same author

Clinical sevoflurane metabolism and disposition. II. The role of cytochrome P450 2E1 in fluoride and hexafluoroisopropanol formation.

Anesthesiology·1995

Same author

Preliminary evaluation of the ligase chain reaction for specific detection of Neisseria gonorrhoeae.

Journal of clinical microbiology·1992

Same author

Sigmoid stricture at colonoscopy--an indication for surgery.

International journal of colorectal disease·1990

Same author

Use of gonozyme on urine sediment for diagnosis of gonorrhea in males.

Journal of clinical microbiology·1986

Area of Science:

Immunology
Microbiology

Background:

Opsonic antibodies are crucial for bacterial clearance.
Traditional bactericidal assays often use cumbersome tube-based methods.

Purpose of the Study:

To evaluate the efficacy of microtiter plates as a substitute for tubes in bactericidal assays for opsonic antibodies.

Main Methods:

Bactericidal assays were performed using microtiter plates instead of traditional tubes.
The assay measured the killing of bacteria in the presence of serum and complement.

Main Results:

Microtiter plates provided a successful platform for the bactericidal test.
The microtiter plate method demonstrated efficiency in evaluating opsonic antibody activity.

Conclusions:

Microtiter plates offer an efficient and viable alternative to tubes for bactericidal assays.
This optimized method facilitates high-throughput screening of sera for opsonic antibody levels.

More Related Videos

A High-throughput Shigella-specific Bactericidal Assay

A High-throughput Shigella-specific Bactericidal Assay

Published on: February 27, 2019

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Published on: April 5, 2019

Related Experiment Videos

Last Updated: Jul 17, 2026

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Published on: April 10, 2016

A High-throughput Shigella-specific Bactericidal Assay

A High-throughput Shigella-specific Bactericidal Assay

Published on: February 27, 2019

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Published on: April 5, 2019