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Internal cellular details of Euglena gracilis visualized by scanning electron microscopy
Science (New York, N.Y.)
|January 22, 1971
Summary
Simple washing and preparation techniques allow scanning electron microscopy of Euglena gracilis. This method reveals detailed internal structures like organelles and endoplasmic reticulum through the cell
Area of Science:
- Cell Biology
- Microscopy Techniques
Background:
- The pellicle of Euglena gracilis typically obscures internal structures.
- External coating material on Euglena gracilis hinders detailed microscopic observation.
Purpose of the Study:
- To develop a method for visualizing internal organelles of Euglena gracilis using scanning electron microscopy.
- To enable direct observation of cellular components through improved sample preparation.
Main Methods:
- Washing live Euglena gracilis to remove external coating.
- Fixing and then mechanically disrupting (breaking or freeze-fracturing) the cells.
- Scanning electron microscopy (SEM) at magnifications of x 5,000 to x 21,000.
Main Results:
- Washing thins the pellicle, allowing organelle outlines to be visible.
- Cell disruption methods reveal internal organelles, storage granules, endoplasmic reticulum, and nuclear pores.
- High magnification SEM provides detailed images with significant depth of focus.
Conclusions:
- A refined sample preparation protocol enhances SEM imaging of Euglena gracilis.
- This technique facilitates detailed ultrastructural analysis of Euglena gracilis organelles.