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Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

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Related Experiment Video

Updated: May 11, 2026

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
10:25

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

Platelet storage at 22 degrees C; metabolic, morphologic, and functional studies.

S Murphy, F H Gardner

    The Journal of Clinical Investigation
    |February 1, 1971
    PubMed
    Summary

    Stored platelets maintain viability and function, with defects reversing after transfusion. This study examines platelet storage lesion, finding reversible changes in platelet metabolism and function.

    Area of Science:

    • Hematology
    • Transfusion Medicine
    • Biochemistry

    Background:

    • Platelets are critical for hemostasis.
    • Optimal storage conditions for platelet transfusion are essential.
    • Understanding the platelet storage lesion is key to improving transfusion efficacy.

    Purpose of the Study:

    • To investigate metabolic, morphologic, and functional changes in stored platelets.
    • To evaluate the reversibility of storage-induced defects after transfusion.

    Main Methods:

    • Metabolic studies (glycogen, ATP, ADP, glucose utilization).
    • Morphologic analysis (electron microscopy, phase microscopy).
    • Functional assays (platelet aggregation).
    • In vivo studies in thrombocytopenic recipients.

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    Microfluidics in Assessing Platelet Function
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    Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

    Published on: January 10, 2025

    Related Experiment Videos

    Last Updated: May 11, 2026

    Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
    10:25

    Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

    Published on: March 19, 2016

    Microfluidics in Assessing Platelet Function
    06:47

    Microfluidics in Assessing Platelet Function

    Published on: November 8, 2024

    Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
    07:06

    Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

    Published on: January 10, 2025

    Main Results:

    • Lactate accumulation occurred during storage without significant changes in pH or glucose.
    • Platelet glycogen decreased, but ATP, ADP, and intracellular potassium remained stable.
    • Platelet aggregation was reduced after storage, yet metabolic pathways remained intact.
    • Transfused platelets showed recovery of glycogen and aggregation capacity within 24 hours.

    Conclusions:

    • Platelets remain largely intact during short-term storage (24 hours to 3-4 days at 22°C).
    • Observed defects, such as reduced aggregation and glycogen depletion, are reversible upon transfusion.
    • The specific "storage lesion" causing irreversible loss of platelet viability was not identified in this study.