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Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
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Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Published on: September 5, 2017

Chromosome lesions produced with an argon laser microbeam without dye sensitization

M W Berns, W K Cheng, A D Floyd

    Science (New York, N.Y.)
    |March 5, 1971
    PubMed

    Abstract:

    Improvements in the argon laser microbeam have made it possible to cause damage to chromosomes of tissue culture cells without prior treatment of the cells with a photosensitizing agent. These results have been confirmed independently in two laboratories.

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    Nucleotide Excision Repair01:46

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    Exposure to mutagens can damage DNA and result in bulky lesions that distort the double-helix structure or impede proper transcription. Damaged DNA can be detected and repaired in a process called nucleotide excision repair (NER). NER employs a set of specialized proteins that first scan DNA to detect a damaged region. Next, NER proteins separate the strands and excise the damaged area. Finally, they coordinate the replacement with new, matching nucleotides.DNA distortion and damageCells are...

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