Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative micro-complement fixation test.

A A Marucci, T C Fuller

    Applied Microbiology
    |February 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    A new quantitative micro-complement fixation test can detect nanogram amounts of antigen. This highly reproducible immunoassay is simple to perform, offering sensitive antigen detection for various systems.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Transplantation of ABO group A2 kidneys from living donors into group O and B recipients.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2002
    Same author

    A strategy for high throughput HLA-DQ typing.

    Journal of immunological methods·2001
    Same author

    Prospective randomized trial of azathioprine in cryopreserved valved allografts in children.

    The Annals of thoracic surgery·2001
    Same author

    A prospective analysis of the immunogenicity of cryopreserved nonvalved allografts used in pediatric heart surgery.

    Circulation·2000
    Same author

    Class I and class II anti-HLA antibodies after implantation of cryopreserved allograft material in pediatric patients.

    The Journal of thoracic and cardiovascular surgery·2000
    Same author

    Repeat donor HLA-DR mismatches in renal transplantation: is the increased failure rate caused by noncytotoxic HLA-DR alloantibodies?

    Transplantation·1999
    Same journal

    Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents.

    Applied microbiology·1975
    Same journal

    Microbial indicators of oil-rich salt marsh sediments.

    Applied microbiology·1975
    Same journal

    Accelerated Fermentation of Brewer's Wort by Saccharomyces carlsbergensis.

    Applied microbiology·1975
    Same journal

    Semisolid fermentation of ryegrass straw.

    Applied microbiology·1975
    Same journal

    Microbiology of ripening honey.

    Applied microbiology·1975
    Same journal

    Microwave sanitization of color additives used in cosmetics: feasibility study.

    Applied microbiology·1975
    See all related articles

    Area of Science:

    • Immunology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Accurate quantification of antigens is crucial in various biological and diagnostic applications.
    • Existing methods for antigen detection may lack sensitivity or reproducibility.

    Purpose of the Study:

    • To describe a novel quantitative micro-complement fixation test.
    • To demonstrate the test's capability for detecting nanogram quantities of antigen.
    • To highlight the test's simplicity and reproducibility.

    Main Methods:

    • Development of a quantitative micro-complement fixation assay.
    • Application of the assay to antigen-antibody systems.
    • Evaluation of test performance, including sensitivity and reproducibility.

    Related Experiment Videos

    Main Results:

    • The micro-complement fixation test successfully detects nanogram quantities of antigen.
    • The assay demonstrates high reproducibility across different antigen-antibody systems.
    • The test is straightforward to implement in a laboratory setting.

    Conclusions:

    • The described micro-complement fixation test offers a sensitive and reproducible method for antigen quantification.
    • This assay provides a valuable tool for immunological and biochemical analyses.
    • The simplicity of the test facilitates its widespread adoption.