Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mannitol utake by Saccharomyces cerevisiae.

Maxwellwa, E Spoerl

    Journal of Bacteriology
    |March 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    Saccharomyces cerevisiae actively transports mannitol via a carrier system, requiring cellular energy. Mannitol uptake is influenced by temperature, inhibitors, and competing sugars, with minimal efflux observed.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Corneal collagen cross-linking with riboflavin and ultraviolet-A light in progressive keratoconus. Results after 10-year follow-up].

    Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2014
    Same author

    Membrane changes in yeast cells caused by sulfhydryl reagents and accompanied by a selective release of sugar.

    The Journal of membrane biology·2013
    Same author

    [Commentary].

    Klinische Monatsblatter fur Augenheilkunde·2013
    Same author

    [Effectivity of an occlusion-supporting PC-based visual training programme by horizontal drifting sinus gratings in children with amblyopia].

    Klinische Monatsblatter fur Augenheilkunde·2012
    Same author

    [Corneal cross-linking with hypo-osmolar riboflavin solution for keratoconus with thin corneas].

    Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2011
    Same author

    [Enzymatic evidence of the depth dependence of stiffening on riboflavin/UVA treated corneas].

    Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2007

    Area of Science:

    • Cellular Biology
    • Biochemistry
    • Microbiology

    Background:

    • Mannitol is a nonmetabolized hexitol relevant to yeast physiology.
    • Understanding nutrient transport mechanisms in Saccharomyces cerevisiae is crucial for various biotechnological applications.

    Purpose of the Study:

    • To investigate the characteristics of mannitol uptake in Saccharomyces cerevisiae.
    • To elucidate the energy requirements and carrier-mediated nature of this transport process.

    Main Methods:

    • Measurement of mannitol uptake under varying temperatures.
    • Assays for inhibition by uranyl nitrate and competition with glucose.
    • Analysis of counterflow kinetics and Michaelis constant determination.
    • Assessment of energy dependence using inhibitors and anaerobic conditions.

    Related Experiment Videos

  • Investigation of intracellular binding and osmotic response.

    • Main Results:

    • Mannitol uptake is temperature-dependent and inhibited by uranyl nitrate.
    • Glucose competes with mannitol for uptake, indicating a shared carrier system.
    • Energy is required for mannitol transport, demonstrated by inhibitor effects and anaerobic conditions.
    • Mannitol binds to intracellular components, leading to minimal efflux and no osmotic response.

    Conclusions:

    • Saccharomyces cerevisiae possesses an active, carrier-mediated system for mannitol uptake.
    • The transport process is energy-dependent and subject to regulation by environmental factors and competing substrates.
    • Intracellular binding contributes to the retention of mannitol within the yeast cell.