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Pluripotential stem cell replication in continuous human, prosimian, and murine bone marrow culture
Blood Cells
|June 15, 1979
Summary
This study shows that murine marrow cultures can sustain stem cell replication long-term. Viral infections altered stem cell behavior, indicating potential for studying leukemogenesis in various species.
Area of Science:
- Hematology
- Virology
- Stem Cell Biology
Background:
- Murine marrow culture systems support prolonged replication of stem and progenitor cells.
- Viral infections can alter hematopoietic stem cell replication and differentiation.
Purpose of the Study:
- To investigate the effects of viral infections on stem cell replication and differentiation in murine marrow cultures.
- To assess the applicability of marrow culture systems for studying in vitro leukemogenesis across species.
Main Methods:
- Utilized a murine marrow culture system to sustain pluripotential stem cells and committed progenitor cells.
- Infected cultures with Friend virus and Kirsten sarcoma virus to observe alterations in cell replication and differentiation.
- Established continuous human and prosimian marrow cultures for comparative analysis.
Main Results:
- Prolonged replication of stem and progenitor cells was sustained in murine marrow cultures.
- Friend virus infection led to pluripotential stem cell transformation.
- Kirsten sarcoma virus infection resulted in transformation of the macrophage component of the hematopoietic microenvironment.
- Prolonged myelopoiesis and CFU-c proliferation were observed in human and prosimian marrow cultures.
Conclusions:
- Murine marrow cultures provide a model for sustained stem cell replication.
- Viral infections can induce specific transformations within the hematopoietic system.
- Marrow culture techniques are applicable for studying stem cell control and in vitro leukemogenesis in humans and other primates.